Directive EU 2018/2001 on the promotion of the use of energy from renewable sources has been officially ratified in December 2018 for the post-2020 framework. It is a new iteration of the Renewable Energy Directive RED, the so-called ‘recast’, work on which began in 2016. The Directive fixes a minimum requirement for greenhouse gas (GHG) savings for biofuels and bioliquids for the period from 2021 to 2030, and sets the rules for calculating the greenhouse impact of biofuels, bioliquids and their fossil fuels comparators. To help economic operators to declare the GHG emission savings of their products, default and typical values for a number of specific pathways are listed in the annexes of the RED-recast (Annex V). The EC Joint Research Center (JRC) is in charge of defining input values to be used for the calculation of default GHG emissions for biofuels, bioliquids, solid and gaseous biomass pathways. An update of the GHG emissions in Annex V has been carried out for the new Directive on the Promotion of the Use of Energy from Renewable Sources (Directive 2018/2001), for the post-2020 framework. This report describes the assumptions made by the JRC when compiling the new updated data set used to calculate default and typical GHG emissions for the different biofuels pathways as proposed in the new directive.